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what factors are involved in the separation of the pigments

What factors affect the separation of pigments? – What factors are involved in the separation of pigments? Solubility, the size of the particles and the pigments attractiveness to the paper based upon the pigment’s chemical structure.

What are the factors that determine the separation of pigments during chromatography? – the factors that affecting the movement of pigment during chromatography were porosity of the chromatography paper,solubility of the solvent as well as the molecular size of the solute.

How do you separate a mixture of plant pigments? – A mixture of plant pigments can be separated by chromatography.

What two factors cause pigments to move at different rates? – The pigments are carried at different rates because they are not equally soluble. A pigment that is the most soluble will travel the greatest distance and a pigment that is less soluble will move a shorter distance.

What are the factors that affect chromatography? – Rf values and reproducibility can be affected by a number of different factors such as layer thickness, moisture on the TLC plate, vessel saturation, temperature, depth of mobile phase, nature of the TLC plate, sample size, and solvent parameters.

How does separation occur in chromatography? – The different components of the mixture travel through the stationary phase at different speeds, causing them to separate from one another. The nature of the specific mobile and stationary phases determines which substances travel more quickly or slowly, and is how they are separated.

How do you extract pigments? – To extract crude pigments, a plant sample is ground and mixed with an extraction solvent, and the mixture is left in an extraction vessel with occasional shaking or stirring.

How are pigments extracted from leaves?

Which technique is used for the separation of leaf pigments which lesson? – In order to view and distinguish the primary four plant pigments, a simple technique known as chromatography can be used.

What factors affect paper chromatography? – › hplc-basics › factors-affecting-rf-…

How does temperature affect chromatography? – Temperature can affect the separation of components in all chromatography types. If the temperature rises, the heat transfers further energy to the solvent-giving the molecule the power to escape from the surface of the liquid hence increases the transfer of liquid to the vapor phase.

Which technique is used for the separation of leaf pigments? – Most leaves are green due to chlorophyll pigments of different types like chlorophyll A, chlorophyll B, etc. All these pigments will be seperated according to their molecular composition. So, the correct option is ‘Paper Chromatography’.

How does solubility affect chromatography? – Higher the solubility of the constituents in the mobile phase, faster will be the mobility rate. Hence, the solubility of the components of the mixture affects the process of chromatography as higher the solubility in the mobile phase, faster the components will elute out of the chromatography stationary phase.


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